peritoneum tissue sections  (OriGene)

Journal: Renal Failure

Article Title: miR-15a-5p suppresses peritoneal fibrosis induced by peritoneal dialysis via targeting VEGF in rats

doi: 10.1080/0886022x.2020.1811123

Figure Lengend Snippet: Figure 1. The rat uremia model was successfully constructed. (A) The serum of rats in each group was collected before periton- eal dialysis. The levels of creatinine (Scr) and blood urea nitrogen (BUN) are shown (p < 0.05). (B) Expression of miR-15a-5p and VEGF in the peritoneum, as detected by quantitative real-time PCR (qRT-PCR). p < 0.05 versus control, #p < 0.05 versus PD.

Article Snippet: Immunohistochemical staining of peritoneum tissue sections was performed using a rabbit two-step detection kit and a DAB developing kit (Goldenbridge Biotechnology Co. Ltd., Beijing, China).

Techniques: Construct, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Journal: Gene therapy

Article Title: TGF-β₁-siRNA delivery with nanoparticles inhibits peritoneal fibrosis.

doi: 10.1038/gt.2014.116

Figure Lengend Snippet: Figure 1. Distribution of siRNAs-NPs in the peritoneum. Immunofluorescence analyses of intraperitoneally injected Cy3-labeled siRNAs-NPs and siRNAs alone in the peritoneum. DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: To evaluate cells that were positive for α-SMA, cytokeratin, CD45 and CD31, sections of pretreated peritoneum tissue were incubated overnight at 4 °C with a monoclonal antibody against mouse α-SMA conjugated to Cy3 (Sigma–Aldrich) at 1:400 dilution, cytokeratin (Acris Antibodies, San Diego, CA, USA) at 1:50 dilution, CD45 (Abcam) at 1:400 dilution or CD31 (Abcam) at 1:50 dilution.

Techniques: Injection, Labeling

Journal: Gene therapy

Article Title: TGF-β₁-siRNA delivery with nanoparticles inhibits peritoneal fibrosis.

doi: 10.1038/gt.2014.116

Figure Lengend Snippet: Figure 2. Knockdown of TGF-β1 expression in the peritoneum and inhibition of TGF-β1 expression in peritoneal drainage fluid by TGF-β1- siRNAs-NPs. (a) Representative immunofluorescence staining and quantitative analyses of TGF-β1-positive cells in peritoneum tissue sections in each group. (b) TGF-β1 concentration was measured by ELISA in the peritoneal drainage fluid in each group. Each group: n = 6, values are the mean ± s.e. (error bars) of at least three independent experiments. DAPI, 4′,6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; NS, not significant; *Po0.05.

Article Snippet: To evaluate cells that were positive for α-SMA, cytokeratin, CD45 and CD31, sections of pretreated peritoneum tissue were incubated overnight at 4 °C with a monoclonal antibody against mouse α-SMA conjugated to Cy3 (Sigma–Aldrich) at 1:400 dilution, cytokeratin (Acris Antibodies, San Diego, CA, USA) at 1:50 dilution, CD45 (Abcam) at 1:400 dilution or CD31 (Abcam) at 1:50 dilution.

Techniques: Knockdown, Expressing, Inhibition, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Gene therapy

Article Title: TGF-β₁-siRNA delivery with nanoparticles inhibits peritoneal fibrosis.

doi: 10.1038/gt.2014.116

Figure Lengend Snippet: Figure 3. Effects of TGF-β1-siRNAs-NPs on peritoneal fibrous thickness. Representative Azan staining of peritoneum tissue and quantitative analyses of peritoneal thickness in each group. Each group: n = 6, values are the mean ± s.e. (error bars) of at least three independent experiments. NS, not significant; *Po0.05; **Po0.01.

Article Snippet: To evaluate cells that were positive for α-SMA, cytokeratin, CD45 and CD31, sections of pretreated peritoneum tissue were incubated overnight at 4 °C with a monoclonal antibody against mouse α-SMA conjugated to Cy3 (Sigma–Aldrich) at 1:400 dilution, cytokeratin (Acris Antibodies, San Diego, CA, USA) at 1:50 dilution, CD45 (Abcam) at 1:400 dilution or CD31 (Abcam) at 1:50 dilution.

Techniques: Staining

Journal: Gene therapy

Article Title: TGF-β₁-siRNA delivery with nanoparticles inhibits peritoneal fibrosis.

doi: 10.1038/gt.2014.116

Figure Lengend Snippet: Figure 5. Effects of TGF-β1-siRNAs-NPs on expression of α-SMA-positive myofibroblasts in the peritoneum. Representative immunofluores- cence (a) and quantitative analyses (b) of α-SMA-positive myofibroblasts in each group. Each group: n = 6, values are the mean ± s.e. (error bars) of at least three independent experiments. DAPI, 4′,6-diamidino-2-phenylindole; NS, not significant; *Po0.05; **Po0.01.

Article Snippet: To evaluate cells that were positive for α-SMA, cytokeratin, CD45 and CD31, sections of pretreated peritoneum tissue were incubated overnight at 4 °C with a monoclonal antibody against mouse α-SMA conjugated to Cy3 (Sigma–Aldrich) at 1:400 dilution, cytokeratin (Acris Antibodies, San Diego, CA, USA) at 1:50 dilution, CD45 (Abcam) at 1:400 dilution or CD31 (Abcam) at 1:50 dilution.

Techniques: Expressing

Journal: Gene therapy

Article Title: TGF-β₁-siRNA delivery with nanoparticles inhibits peritoneal fibrosis.

doi: 10.1038/gt.2014.116

Figure Lengend Snippet: Figure 6. Effects of TGF-β1-siRNAs-NPs on myofibroblasts derived from multiple origins. Representative immunohistochemistry of cells double- positive (yellow) for cytokeratin and α-SMA (a) and for CD45 and α-SMA (b), and quantitative analyses of cells double-positive for cytokeratin and α-SMA (c) and for CD45 and α-SMA (d), and cells single-positive for α-SMA (e) in the peritoneum in each group. Each group: n = 4, values are the mean ± s.e. (error bars) of at least two independent experiments. DAPI, 4′,6-diamidino-2-phenylindole; NS, not significant; *Po0.05; **Po0.01.

Article Snippet: To evaluate cells that were positive for α-SMA, cytokeratin, CD45 and CD31, sections of pretreated peritoneum tissue were incubated overnight at 4 °C with a monoclonal antibody against mouse α-SMA conjugated to Cy3 (Sigma–Aldrich) at 1:400 dilution, cytokeratin (Acris Antibodies, San Diego, CA, USA) at 1:50 dilution, CD45 (Abcam) at 1:400 dilution or CD31 (Abcam) at 1:50 dilution.

Techniques: Derivative Assay, Immunohistochemistry